One year's experience with two different image analysis systems for automated reading of the contrast fluorescence test

Area of Science:

• Immunology
• Medical Laboratory Science
• Biotechnology

Background:

• The microlymphocytotoxicity test (LCT) is a standard method for HLA-A,B,C typing and screening.
• Visual reading of LCT results can be subjective and labor-intensive.
• Automated systems are needed to improve efficiency and objectivity in LCT analysis.

Purpose of the Study:

• To evaluate two personal-computer-based color image analysis systems for automated LCT reading.
• To assess the feasibility and performance of digital image analysis for HLA typing.

Main Methods:

• Over 17,000 microlymphocytotoxicity reactions were performed using the contrast fluorescence test (CFT) variant.
• Tests were visually read by experienced staff and also analyzed using automated scanning and digital image analysis systems.
• Color CCD videocamera recorded reactions for subsequent analysis by specifically developed programs.

Main Results:

• A strong correlation (r = 0.89) was observed between digital image analysis scores and visual tray readings.
• The prototype digital image analysis system demonstrated acceptable performance characteristics for routine application.

Conclusions:

• Digital image analysis is a feasible and promising technique for the automated evaluation of the microlymphocytotoxicity test.
• This technology has the potential to enhance the efficiency and objectivity of HLA typing and screening.