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beta(1-3)Glucanosyltransferase Gel4p is essential for Aspergillus fumigatus | JoVE Visualize

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beta(1-3)Glucanosyltransferase Gel4p is essential for Aspergillus fumigatus

Amandine Gastebois¹, Thierry Fontaine, Jean-Paul Latgé

¹Unité des Aspergillus, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France.

Eukaryotic Cell|June 15, 2010

View abstract on PubMed

Summary

The Aspergillus fumigatus GEL4 gene is essential for fungal growth, unlike other related beta-(1,3)-glucanosyltransferases. This study investigated the role of this crucial gene in the fungus.

Area of Science:

Mycology
Molecular Biology
Biochemistry

Background:

Aspergillus fumigatus possesses a family of 7 beta-(1,3)-glucanosyltransferase (Gel) genes.
Only 3 of these genes are expressed during the fungus's mycelial growth phase.
The GEL4 gene encodes a glycosylphosphatidylinositol (GPI)-anchored protein, similar to other characterized Gelps.

Purpose of the Study:

To investigate the specific role of the GEL4 gene in Aspergillus fumigatus.
To determine the essentiality of GEL4 for fungal growth and survival.

Main Methods:

Gene expression analysis during mycelial growth.
Characterization of the protein product of the GEL4 gene.
Functional analysis of GEL4 in Aspergillus fumigatus.

Main Results:

The GEL4 gene is expressed during mycelial growth in Aspergillus fumigatus.
GEL4 encodes a GPI-anchored protein.
Unlike other beta-(1,3)-glucanosyltransferases, GEL4 is essential for Aspergillus fumigatus.

Conclusions:

The GEL4 gene plays a critical and essential role in the growth of Aspergillus fumigatus.
GEL4 represents a unique and vital enzyme within the beta-(1,3)-glucanosyltransferase family in this fungal species.

Other Engineering

Granular Mechanics

Beta(1-3)glucanosyltransferase Gel4p Is Essential For Aspergillus Fumigatus