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Sodium-translocating adenosine triphosphatase in Streptococcus faecalis | JoVE Visualize

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Sodium-translocating adenosine triphosphatase in Streptococcus faecalis

Y Kakinuma¹, K Igarashi

¹Faculty of Pharmaceutical Sciences, Chiba University, Japan.

Journal of Bioenergetics and Biomembranes|December 1, 1989

View abstract on PubMed

Summary

This study reveals that sodium-translocating ATPase in Streptococcus faecalis is induced by high sodium levels, suggesting a role in managing sodium-rich environments. The enzyme

Area of Science:

Microbiology
Biochemistry
Molecular Biology

Background:

Streptococcus faecalis possesses a sodium-translocating ATPase.
This enzyme exchanges sodium ions for potassium ions.
Sodium ions stimulate ATPase activity, while potassium ions do not.

Purpose of the Study:

To investigate the regulation and characteristics of the sodium-translocating ATPase in Streptococcus faecalis.
To understand the enzyme's role in cellular ion homeostasis and adaptation to environmental conditions.

Main Methods:

The study involved growing Streptococcus faecalis in media with varying sodium concentrations.
Enzyme activity was measured under different ionic conditions.
Resistance to specific inhibitors (vanadate, dicyclohexylcarbodiimide) was assessed to differentiate the enzyme from other ATPases.

Main Results:

The sodium ATPase is induced when cells are grown in sodium-rich media.
Induction is particularly pronounced when proton potential generation is limited or the sodium-proton antiporter is blocked.
The enzyme is not induced by potassium deprivation.
The enzyme exhibits resistance to vanadate and dicyclohexylcarbodiimide, distinguishing it from E1E2 and F1F0 type ATPases.

Conclusions:

Increased cytoplasmic sodium levels act as a signal for sodium ATPase induction.
The sodium ATPase likely evolved to help bacteria cope with sodium-rich environments, especially when proton potential is compromised.
This enzyme plays a crucial role in maintaining ion balance and survival under specific environmental stresses.

Synchrotrons And Accelerators

Sodium-translocating Adenosine Triphosphatase In Streptococcus Faecalis