Long-term stabilization of hydrogen peroxide by poly(vinyl alcohol) on paper-based analytical devices | JoVE Visualize

Long-term stabilization of hydrogen peroxide by poly(vinyl alcohol) on paper-based analytical devices

Tuchpongpuch Boonpoempoon¹, Wanida Wonsawat¹, Takashi Kaneta²

¹Department of Chemistry, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand.

Scientific Reports|September 12, 2019

View abstract on PubMed

Summary

Poly(vinyl alcohol) (PVA) stabilizes hydrogen peroxide on paper-based analytical devices (PADs). This improves the long-term stability of diagnostic tests, crucial for point-of-care applications.

Area of Science:

Analytical Chemistry
Materials Science
Biochemistry

Background:

Paper-based analytical devices (PADs) are vital for point-of-care testing.
Ensuring the long-term stability of reagents on PADs is a significant challenge.
Degradation of reagents like hydrogen peroxide limits PAD shelf-life.

Purpose of the Study:

To investigate the stabilizing effect of poly(vinyl alcohol) (PVA) on hydrogen peroxide on paper substrates.
To evaluate PVA's impact on the stability of PADs for diagnostic applications.
To determine optimal PVA concentrations and storage conditions for enhanced reagent stability.

Main Methods:

Colorimetric measurements using horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine as substrates.
Preparation of PADs with varying concentrations of PVA (0.5-2%) and hydrogen peroxide.
Comparative stability studies of hydrogen peroxide with and without PVA under ambient and refrigerated conditions.

Main Results:

Poly(vinyl alcohol) significantly suppressed the degradation of hydrogen peroxide on paper.
PVA concentrations between 0.5% and 2% enhanced hydrogen peroxide stability, with 1% PVA showing comparable results to 2%.
Refrigerated storage (4°C) of PADs with 2% PVA extended hydrogen peroxide stability up to 30 days, compared to degradation within one day at room temperature without PVA.

Conclusions:

Poly(vinyl alcohol) is an effective stabilizing reagent for hydrogen peroxide in paper-based analytical devices.
PVA incorporation enhances the shelf-life of PADs, making them more reliable for point-of-care diagnostics.
Optimized PVA concentration and cold storage can significantly extend the operational stability of PADs.