Optimized Production of the Allylamine Antifungal "Terbinafine" by Lysinibacillus Isolate MK212927 Using Response Surface Methodology

Area of Science:

• Microbiology
• Biotechnology
• Medicinal Chemistry

Background:

• Terbinafine is a broad-spectrum allylamine antifungal agent.
• Natural microbial sources offer potential for sustainable antifungal production.
• Lysinibacillus species are known producers of bioactive compounds.

Purpose of the Study:

• To optimize terbinafine production by Lysinibacillus isolate MK212927.
• To identify key factors influencing antifungal metabolite yield.
• To enhance the natural production of terbinafine.

Main Methods:

• Central composite design was used for optimization.
• Five variables were investigated: carbon source, nitrogen source, temperature, pH, and agitation.
• Terbinafine production was quantified using inhibition zone diameter and a calibration curve.

Main Results:

• Optimal conditions identified: starch 5 g/L, ammonium chloride 5 g/L, 32°C, 150 rpm, pH 7.
• Optimized conditions increased terbinafine production by approximately 1.6-fold (1814.662 µg/mL).
• The predictive model showed high accuracy, validating the optimization process.

Conclusions:

• This is the first report on optimized terbinafine production by Lysinibacillus species.
• The findings provide baseline data for scaling up terbinafine production from microbial sources.
• This research contributes to the sustainable supply of a valuable antifungal agent.