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CRISPR-Cas systems: Challenges and future prospects | JoVE Visualize

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CRISPR-Cas systems: Challenges and future prospects

Nisarg Gohil¹, Gargi Bhattacharjee¹, Navya Lavina Lam²

¹Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India.

Progress in Molecular Biology and Translational Science|May 3, 2021

View abstract on PubMed

Summary

Clustered regularly interspaced short palindromic repeats (CRISPR-Cas) systems offer revolutionary genome editing capabilities, surpassing older technologies. However, challenges like off-target effects and delivery methods require further research for full implementation.

Area of Science:

Biotechnology
Molecular Biology
Genetic Engineering

Background:

CRISPR-Cas systems have revolutionized synthetic biology, therapeutics, diagnostics, and metabolic engineering.
These systems offer precise, rapid, cost-effective, and efficient genome editing, surpassing previous zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) technologies.

Purpose of the Study:

To present and discuss the obstacles faced in the implementation of CRISPR-Cas systems.
To explore the future prospects of CRISPR-Cas technology.

Main Methods:

Review and analysis of current CRISPR-Cas system applications and limitations.
Identification of key challenges including off-target activity, delivery methods, and ethical/regulatory issues.

Main Results:

CRISPR-Cas technology represents a significant advancement over ZFN and TALEN.
Unresolved challenges include off-target mutations, efficient delivery systems, and ethical considerations.

Conclusions:

CRISPR-Cas systems hold immense potential but require further development to overcome existing hurdles.
Addressing these challenges is crucial for the widespread and responsible application of CRISPR-Cas technology in various fields.

Keywords:

Distributed Computing And Systems Software

Concurrent/parallel Systems And Technologies

Crispr-cas Systems: Challenges And Future Prospects