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Ventilation of a rat with a large-animal respirator | JoVE Visualize

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Ventilation of a rat with a large-animal respirator

J D Wood, N L Herman, D R Kostreva

Journal of Applied Physiology (Bethesda, Md. : 1985)|September 1, 1986

View abstract on PubMed

Summary

This study details a modified tracheostomy tube and Bird Mark 7 respirator system for effectively ventilating rats. This method successfully maintained normal arterial blood gases in rats for over three hours.

Area of Science:

Physiology
Respiratory Medicine
Animal Research

Background:

Maintaining stable physiological parameters is crucial for rodent research.
Effective artificial ventilation techniques are essential for long-term animal studies.

Purpose of the Study:

To describe a novel method for artificial ventilation in rats.
To ensure adequate arterial blood gases (ABGs) are maintained during prolonged procedures.

Main Methods:

A modified tracheostomy tube was created using rubber pilot tubing and polyethylene catheter.
The custom tube was inserted into the trachea of Sprague-Dawley rats (250-500g).
Ventilation was performed using a Bird Mark 7 respirator with initial settings at minimum flow and rate, adjusted based on ABG measurements.

Main Results:

The described ventilation system effectively maintained arterial blood gases within normal limits.
Adequate physiological parameters were sustained for over 3 hours in anesthetized rats.

Conclusions:

This modified tracheostomy tube and Bird Mark 7 respirator system provides a reliable method for prolonged artificial ventilation in rats.
The technique facilitates stable physiological conditions necessary for complex or extended experimental protocols in rodent models.

Mechanical Engineering

Microelectromechanical Systems (mems)

Ventilation Of A Rat With A Large-animal Respirator