ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Purification of the enhancing factor from mouse intestines | JoVE Visualize

Home
Research Domains
Engineering

Purification of the enhancing factor from mouse intestines

R Mulherkar, A Saraf, A Wagle

FEBS Letters|October 20, 1986

View abstract on PubMed

Summary

Researchers isolated and purified a unique enhancing factor (EF) from mouse intestines. This protein boosts the binding of epidermal growth factor (EGF) to cells, confirming its in vitro properties.

Area of Science:

Biochemistry
Cell Biology
Protein Chemistry

Background:

Epidermal Growth Factor (EGF) plays a crucial role in cellular processes.
Enhancing Factor (EF) is a unique polypeptide that modulates EGF binding to cells.
Previous studies partially purified EF using gel-permeation chromatography.

Purpose of the Study:

To achieve homogeneous purification of Enhancing Factor (EF).
To confirm the in vitro properties of purified EF.
To characterize the final purification step of EF.

Main Methods:

Isolation of acid-soluble proteins from mouse intestines.
Purification of EF using high-performance liquid chromatography (HPLC) with a reverse-phase column (mu Bondapak C18).
Confirmation of protein purity using HPLC and SDS-PAGE.

Main Results:

EF was purified to homogeneity.
HPLC analysis showed a single peak, indicating high purity.
SDS-PAGE revealed a single protein band.
Purified EF exhibited consistent in vitro properties compared to partially purified EF.

Conclusions:

Homogeneous purification of Enhancing Factor (EF) was successfully achieved.
HPLC is an effective method for the final purification of EF.
The purified EF retains its biological activity in vitro, validating its role in modulating EGF binding.

Biomedical Engineering

Tissue Engineering

Purification Of The Enhancing Factor From Mouse Intestines