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Transport of glycerol by Pseudomonas aeruginosa | JoVE Visualize

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Transport of glycerol by Pseudomonas aeruginosa

S S Tsay, K K Brown, E T Gaudy

Journal of Bacteriology|October 1, 1971

View abstract on PubMed

Summary

Pseudomonas aeruginosa utilizes a glycerol-binding protein for glycerol transport, which is induced by glycerol and potentially involves facilitated diffusion or active transport mechanisms.

Area of Science:

Microbiology
Molecular Biology
Biochemistry

Background:

Glycerol transport in Pseudomonas aeruginosa is essential for nutrient uptake.
Previous studies indicated genetic control and glycerol induction of this process.

Purpose of the Study:

To elucidate the mechanism of glycerol transport in Pseudomonas aeruginosa.
To identify the molecular components involved in glycerol uptake.

Main Methods:

Utilized radiolabeled glycerol ((14)C-glycerol) to study transport kinetics.
Employed osmotic shock to isolate periplasmic proteins.
Performed equilibrium dialysis to assess glycerol-binding activity.
Investigated the effect of inhibitors (sodium azide, N-ethylmaleimide) and heat treatment.

Main Results:

Glycerol transport is dependent on glycerol induction and a functional transport system.
Evidence suggests two distinct glycerol transport systems with varying affinities.
A glycerol-binding protein was identified in osmotically shocked cells, absent in mutants and uninduced cells.
This binding protein is distinct from glycerol kinase and sensitive to heat, azide, and N-ethylmaleimide.

Conclusions:

Glycerol transport in P. aeruginosa involves a periplasmic glycerol-binding protein crucial for recognition.
The transport mechanism may operate via facilitated diffusion or active transport, with energy requirements not definitively demonstrated.
The findings provide insights into the molecular basis of nutrient transport in bacteria.

Chemical Engineering

Process Control And Simulation

Transport Of Glycerol By Pseudomonas Aeruginosa