ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Characterization of IS46, an insertion sequence found on two IncN plasmids | JoVE Visualize

Home
Research Domains
Information And Computing Sciences

Characterization of IS46, an insertion sequence found on two IncN plasmids

A M Brown, G M Coupland, N S Willetts

Journal of Bacteriology|August 1, 1984

View abstract on PubMed

Summary

The insertion sequence IS46 facilitates plasmid cointegrate formation and resolution in a RecA-dependent manner. It also mediates deletions and inversions, contributing to plasmid evolution and the formation of new plasmids like pKM101.

Area of Science:

Molecular Biology
Genetics
Microbiology

Background:

IncN plasmids R46 and N3 harbor two copies of the insertion sequence IS46.
IS46 is 0.81 kilobases long and possesses single PstI and SalI restriction sites.

Purpose of the Study:

To investigate the transpositional capabilities and recombination functions of IS46.
To understand the role of IS46 in plasmid evolution and the formation of cointegrates, deletions, and inversions.

Main Methods:

Analysis of cointegrate formation mediated by IS46.
Testing the RecA-dependence of IS46-mediated recombination.
Comparison of IS46 homology with other insertion sequences (IS1, IS15).

Main Results:

All four IS46 copies mediated cointegrate formation, but not compound transposon formation.
IS46-mediated cointegrate resolution required Rec+ cells, not RecA- cells.
Recombination between IS46 copies caused inversions in R46, and IS46-mediated deletions likely formed pKM101 from R46. IS46 showed homology with IS15 but not IS1.

Conclusions:

IS46 is an active insertion sequence involved in various DNA rearrangements.
IS46 plays a significant role in the structural evolution of plasmids and the generation of genetic diversity.

Software Engineering

Software Architecture

Characterization Of Is46, An Insertion Sequence Found On Two Incn Plasmids