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Patterns of lactate dehydrogenase isoenzymes during gonadogenesis in the rat | JoVE Visualize

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Biological Sciences

Patterns of lactate dehydrogenase isoenzymes during gonadogenesis in the rat

S Sarkar, A K Dubey, A P Banerji

Journal of Reproduction and Fertility|July 1, 1978

View abstract on PubMed

Summary

Lactate dehydrogenase X (LDH-X) isoenzymes are present in male gonads after birth, specifically in spermatocytes. These isoenzymes were not detected in female gonads or other male reproductive cell types, indicating a sex-specific role.

Area of Science:

Reproductive Biology
Biochemistry
Enzymology

Background:

Lactate dehydrogenase (LDH) exists as various isoenzymes.
LDH isoenzymes play roles in cellular energy metabolism.
Specific LDH isoenzymes are associated with reproductive tissues.

Purpose of the Study:

To investigate the presence and developmental patterns of LDH isoenzymes in gonads.
To identify specific LDH isoenzymes in different testicular cell types.
To determine the sex-specific expression of LDH isoenzymes during development.

Main Methods:

Polyacrylamide gel electrophoresis was used to detect LDH isoenzymes.
Analysis was performed on gonadal tissue from different developmental stages.
Specific cell fractions (spermatocytes, Sertoli cells, Leydig cells, ovary) were isolated and analyzed.

Main Results:

M and H LDH subunits were detected in both male and female gonads from 14.5 days post-coitum (p.c.), with variations during gestation.
Two distinct LDH-X isoenzyme bands (LDH-X1 and LDH-X2) were identified in the spermatocyte-rich fraction from day 20 after birth.
LDH-X isoenzymes were absent in Sertoli cells, Leydig cells, and ovarian tissue.

Conclusions:

LDH-X isoenzymes are specifically expressed in male germ cells (spermatocytes) post-natally.
The expression pattern suggests a specialized role for LDH-X in male gamete development.
LDH-X is a sexually dimorphic marker in gonadal tissue.

Developmental Genetics (incl. Sex Determination)

Patterns Of Lactate Dehydrogenase Isoenzymes During Gonadogenesis In The Rat